Neurohormonal Techniques in Insects

1 Proctolin: Bioassay Isolation and Structure. - I. Introduction. - II. Bioassay. - III. Chemistry. - References. - 2 Adipokinetic Hormone. - I. Introduction. - II. Biological (Bioassay). - III. Chemical. - References. - 3 Bioassay of Diuretic Hormone in Rhodnius. - I. Introduction. - II. Isolation of Malpighian Tubules from Rhodnius. - References. - 4 Diuretic HormoneExtraction and Chemical Properties. - I. Introduction. - II. Assay of Hormone Activity. - III. Isolation of Diuretic Hormone Storage Tissue. - IV. Methods of Fractionation. - V. High K+ Release of Diuretic Hormone In Vitro. - VI. Properties. - VII. Cross-Reactivity of Insect Diuretic Hormones. - VIII. Concluding Remarks. - Acknowledgements. - References. - 5 Insulin-like and Glucagon-like Hormones in Insects. - I. Introduction. - II. Preparation of Tissue Extract. - III. Purification of Extract and Heterogeneity. - IV. Biological Assay. - V. Radioimmunoassay. - VI. Immunocytochemistry. - VII. Concluding Remarks. - Acknowledgement. - References. - Note Added in Proof. - 6 Bursicon. - I. Introduction. - II. Purification of Bursicon. - III. Assays for Bursicon Activity. - References. - 7 Neurohormonal Factors Involved in the Control of Pupariation. - I. What are the Pupariation Factors?. - II. Choice of Material. - III. Breeding Technique. - IV. Staging of the Larvae for Experiments on Pupanation. - V Methods of Observing and Recording Pupariation. - VI Bioassays for the Activity of the Pupariation Factors. - VII. Materials Possessing Activity of the Pupariation Factors. - VIII Chemical Identification of the Pupariation Factors. - IX. Remarks to the Mode of Action of the Pupariation Factors. - References. - 8 Cuticle Plasticizing Factors. - I. Introduction. - II. Bioassay. - III. Chemistry. - References. - 9 Eclosion Hormones. - I. Introduction. - II. Bioassay. -III. Chemistry: Isolation and Purification. - IV. Properties. - V. Biological Activity of the Purified Hormone. - Acknowledgements. - References. - 10 Diapause Hormones. - I. Introduction. - II. Materials. - III. Bioassay. - IV. Extraction. - V. Chromatographic Separation. - VI. Selective Extraction. - VII. Chromatography on Merckogel OR. - VIII. Isolation of DH-A and DH-B. - IX. Molecular Weight 233. - X. Stability of DH in Relation to Degree of Purity. - XL Activity of the Two Species. - XII. Stability and Characters. - XIII. Infrared Spectra. - XIV. Constituents. - Acknowledgments. - References. - 11 Prothoracicotropic Hormone. - I. Introduction. - II. What is Known?-Biological. - III. Bioassay. - IV. Chemistry. - References. Language: English